Screens for mitochondrial function in yeast identified compounds that increase the mitochondrial membrane potential and adenosine triphosphate (ATP) levels. Secondary testing with myotubes, fibroblasts, and PC-12 and HepG2 cells identified compounds increasing ATP levels in hepatocytes and compounds increasing ATP in fibroblasts.

Compounds are from TimTec screening collections: Diveristy SET, NDL-3000, and NPL.

Available identified hits and related compounds from primary and secondary screens described in the publication. Download Excel file with structural information

ID numbers

ST003704
ST003709
ST003710
ST003711
ST003713
ST005192
ST005213
ST006407
ST008330
ST008364
ST008365
ST018585
ST019694
ST019697
ST024021
ST029445
ST029634
ST030891
ST030931
ST037386
ST037803
ST038005
ST038642
ST038645
ST038830
ST049545
ST052057
ST052484
ST053241
ST053256
ST081858

 

Price per compound is 0.1mg/$29.00, 0.25mg/$36.00, 0.5mg/$45.00, 1mg/$55.00, 2mg/$77.00, plus shipping from DE, USA. Please e-mail selected ID numbers for discounted quote for multiple samples purchase. Custom formatting is available.

Currently unavailable samples:

  • ST003707
  • ST003712
  • ST032288
  • ST032694

Reference

Montague CR, Fitzmaurice A,et.al. Screen for Small Molecules Increasing the Mitochondrial Membrane Potential. J Biomol Screen. 2013 Jul 18.

Abstract

The identification of small molecules that positively modulate the mitochondrial respiratory function has broad applications in fundamental  research, therapeutic target validation, and drug discovery. We present an approach in which primary screens for mitochondrial function in  yeast are used to efficiently identify a subset of high-value compounds that can in turn be rapidly tested against a broad range of  mammalian cell lines. The ability of the yeast assay to successfully identify in a high-throughput format hit compounds that increase the  mitochondrial membrane potential and adenosine triphosphate (ATP) levels by as little as 15% was demonstrated. In this study, 14 hits were  identified from a collection of 13,680 compounds. Secondary testing with myotubes, fibroblasts, and PC-12 and HepG2 cells identified two  compounds increasing ATP levels in hepatocytes and two other compounds increasing ATP in fibroblasts. The effect on hepatocytes was further  studied using genomic and mitochondrial proteomic tools to characterize the changes induced by the two compounds. Changes in the  accumulation of a series of factors involved in early gene response or apoptosis or linked to metabolic functions (i.e., ß-Klotho, RORa,  PGC-1a, G6PC, IGFBP1, FTL) were discovered.

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